Highlights
- •We typed aged bone samples with ancestry informative 46 Indels kit.
- •Bone samples were collected from Sub-Saharan Africa, America, East Asia and Europe.
- •AIM Indels successfully estimated the proportion of ancestry of the aged samples.
Abstract
Ancestry informative markers (AIMs) can be useful to infer ancestry proportions of
the donors of forensic evidence. The probability of success typing degraded samples,
such as human skeletal remains, is strongly influenced by the DNA fragment lengths
that can be amplified and the presence of PCR inhibitors. Several AIM panels are available
amongst the many forensic marker sets developed for genotyping degraded DNA. Using
a 46 AIM Insertion Deletion (Indel) multiplex, we analyzed human skeletal remains
of post mortem time ranging from 35 to 60 years from four different continents (Sub-Saharan
Africa, South and Central America, East Asia and Europe) to ascertain the genetic
ancestry components. Samples belonging to non-admixed individuals could be assigned
to their corresponding continental group. For the remaining samples with admixed ancestry,
it was possible to estimate the proportion of co-ancestry components from the four
reference population groups. The 46 AIM Indel set was informative enough to efficiently
estimate the proportion of ancestry even in samples yielding partial profiles, a frequent
occurrence when analyzing inhibited and/or degraded DNA extracts.
Keywords
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Article info
Publication history
Published online: December 05, 2014
Accepted:
November 28,
2014
Received in revised form:
October 25,
2014
Received:
May 29,
2014
Identification
Copyright
© 2014 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
