Highlights
- •Analysis of Danes, Greenlanders and Somalis with Yfiler® Plus PCR amplification kit.
- •Comparisons of results with AmpFlSTRYfiler® PCR amplification kit.
- •Increase in power of discrimination with Yfiler® Plus.
Abstract
Recently, the Yfiler® Plus PCR Amplification Kit (Yfiler® Plus, Thermo Fisher Scientific, Waltham, MA, USA) was introduced. Yfiler® Plus amplifies 27 Y-chromosomal short tandem repeat loci (Y-STRs) and adds ten new
Y-STRs to those analysed with the commonly used AmpFlSTR® Yfiler® PCR Amplification Kit (Yfiler®, Thermo Fisher Scientific, Waltham, MA, USA). Seven of the new Y-STRs are rapidly
mutating Y-STRs (RM Y-STRs). In this study, 551 male individuals from Denmark, Greenland
and Somalia were typed with Yfiler® Plus. The results were compared to those obtained with Yfiler® in the same individuals. Forensic and population genetic parameters were estimated
for Yfiler® Plus. Yfiler® Plus had a higher power of discrimination than Yfiler® in all three populations. Compared to Yfiler®, Yfiler® Plus offers increased power of discrimination, which is obviously an advantage in
crime case investigations. However, the inclusion of seven RM Y-STRs in Yfiler® Plus makes it less attractive for relationship testing because of the relatively
high combined mutation rate, approximately 15%.
Keywords
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Article info
Publication history
Published online: February 17, 2015
Accepted:
February 15,
2015
Received in revised form:
February 9,
2015
Received:
December 19,
2014
Identification
Copyright
© 2015 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
