Highlights
- •The haplotypes of 40 Y-STRs of 1128 unrelated males from 6 Chinese populations.
- •The Y-STR haplotypes are highly polymorphic and have a high discriminating power.
- •Population substructure correction is not necessary due to small Fst and Gst values.
Abstract
Forty Y-STR loci were analyzed in 1128 males from the following six Chinese ethnic
populations: Han (n = 300), Hui (n = 244), Korean (n = 100), Mongolian (n = 100), Uighur (n = 284) and Tibetan (n = 100), utilizing two new generation multiplex Y-STR systems, AGCU Y24 STR and GFS Y24
STR genotyping kits, which allow for the genotyping of 24 loci from a single amplification
reaction in each system. The lowest estimates of genetic diversity (below 0.5) correspond
to markers DYS391 (0.441658) and DYS437 (0.496977), and the greatest diversity corresponds
to markers DYS385a/b (0.969919) and DYS527a/b (0.94676). A considerable number of
duplicate and off-ladder alleles were also revealed. Additionally, there were 1111
different haplotypes identified from the total 1128 samples, of which 1095 were unique.
Notably, no shared haplotypes between populations were observed. The estimated overall
haplotype diversity (HD) was 0.999085, and its discrimination capacity (DC) was 0.970745.
An MDS plot based on the genetic distances between populations showed the genetic
similarity of the southern Han population to the Northern populations of Hui, Korean,
Mongolian and Uighur and a clear genetic departure of the Tibetan population from
other populations. For the Y STR markers, population substructure correction was considered
when calculating the rarity of the Y STR profile. However, because the haplotype based
Fst values are extremely small within the present data (0.000153 with 40 Y-STRs), no
substructure correction is required to estimate the rarity of a haplotype comprising
40 markers. In summary, the results of our study indicate that the 40 Y-STRs have
a high level of polymorphism in Chinese ethnic groups and could therefore be a powerful
tool for forensic applications and population genetic studies.
Keywords
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Article info
Publication history
Published online: August 25, 2015
Accepted:
August 22,
2015
Received in revised form:
August 19,
2015
Received:
May 29,
2015
Identification
Copyright
© 2015 Elsevier Ireland Ltd. Published by Elsevier Inc. All rights reserved.
