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Developmental validation of forensic DNA-STR kits: Expressmarker 16+10Y and expressmarker 16+18Y

      Highlights

      • EX16 + 10Y and EX16 + 18Y PCR systems which combine autosomal and Y chromosome STR loci.
      • The two systems are suitable for individual identification, paternity testing, paternal lineage testing and mixture identification.
      • The two systems provide a new approach for fighting against crime quickly.

      Abstract

      DNA-STR analysis is widely used in the forensic science field and obtaining results in shorter time is highly demanded. The developed forensic STR Kit, referred to as Expressmarker 16 + 10Y (EX16 + 10Y) and Expressmarker 16 + 18Y (EX16 + 18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously. The kits are validated by a series of tests, including DNA mixtures, stutter ratios, PCR based studies, species specificities, inhibitors, sensitivity, sizing precision, reproducibility and parallel tests. The results demonstrated that EX16 + 10Y and EX16 + 18Y were useful tools for rapid criminal investigation.

      Keywords

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