Highlights
- •EX16 + 10Y and EX16 + 18Y PCR systems which combine autosomal and Y chromosome STR loci.
- •The two systems are suitable for individual identification, paternity testing, paternal lineage testing and mixture identification.
- •The two systems provide a new approach for fighting against crime quickly.
Abstract
DNA-STR analysis is widely used in the forensic science field and obtaining results
in shorter time is highly demanded. The developed forensic STR Kit, referred to as
Expressmarker 16 + 10Y (EX16 + 10Y) and Expressmarker 16 + 18Y (EX16 + 18Y), could amplify the common autosomal and Y chromosome STR loci simultaneously.
The kits are validated by a series of tests, including DNA mixtures, stutter ratios,
PCR based studies, species specificities, inhibitors, sensitivity, sizing precision,
reproducibility and parallel tests. The results demonstrated that EX16 + 10Y and EX16 + 18Y were useful tools for rapid criminal investigation.
Keywords
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Article info
Publication history
Published online: May 17, 2016
Accepted:
May 14,
2016
Received in revised form:
May 9,
2016
Received:
November 16,
2015
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
