- •We evaluated five DNA preservatives (modified TENT, DESS, LST, RNAlater and DNAgard®) on decomposing tissues stored in hot and humid conditions.
- •A marked decrease in DNA quantity and quality was observed in all samples after the bodies entered the bloat stage of decomposition.
- •Similar amounts of DNA were retrieved from skin and muscle samples over time, but slightly more complete STR profiles were obtained from muscle tissue.
- •Although higher amounts of DNA were recovered from tissue samples, the completeness of STR profiles from “free DNA” in solution was comparable.
- •DNAgard® and the modified TENT buffer were the most successful preservatives based on STR success from “free DNA” in solution.
Purchase one-time access:Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
One-time access price info
- For academic or personal research use, select 'Academic and Personal'
- For corporate R&D use, select 'Corporate R&D Professionals'
Subscribe:Subscribe to Forensic Science International: Genetics
- DNA Commission of the International Society for Forensic Genetics (ISFG): Recommendations regarding the role of forensic genetics for disaster victim identification (DVI).FSI: Genet. 2007; 1: 3-12
- Room temperature DNA preservation of soft tissue for rapid DNA extraction: an addition to the disaster victim identification investigators toolkit?.FSI: Genet. 2008; 2: 29-34
- Long-term room temperature preservation of corpse soft tissue: an approach for tissue sample storage.Invest. Genet. 2011; 2
- An optimized protocol for forensic application of the PreCR™ Repair Mix to multiplex STR amplification of UV-damaged DNA.FSI: Genet. 2012; 6: 498-503
- Forensic implications of genetic analyses from degraded DNA—a review.FSI: Genet. 2010; 4: 148-157
- Challenging DNA: Assessment of a range of genotyping approaches for highly degraded forensic samples.FSI: Genet. Suppl. Ser. 2008; 1: 26-28
- Quantifiler Trio Kit and forensic samples management: a matter of degradation.FSI: Genet. 2015; 16: 77-85
- Development of a real-time method to detect DNA degradation in forensic samples.J. Forensic Sci. 2012; 57: 466-471
- Human tissue preservation for disaster victim identification (DVI) in tropical climates.FSI: Genet. 2012; 6: 653-657
Coleman J, U.S. Department Of Justice, Federal Bureau of Investigation. Handbook of Forensic Services, 2013. https://www.fbi.gov/about-us/lab/handbook-of-forensic-services-pdf
- AmpFlSTR® Profiler Plus™ and AmpFlSTR® COfiler™ analysis of tissues stored in GenoFix™, a new tissue preservation solution for mass disaster DNA identification.J. Forensic Sci. 2001; 46: 1180-1190
- Comparison of DNA preservation methods for environmental bacterial community samples.FEMS Microbiol. Ecol. 2013; 83: 468-477
- Simplified field preservation of tissues for subsequent DNA analyses.J. Forensic Sci. 2011; 56: 846-852
- Assessing biodiversity of a freshwater benthic macroinvertebrate community through non-destructive environmental barcoding of DNA from preservative ethanol.BMC Ecol. 2012; 12
Qiagen, MinElute® Handbook, June 2004. https://www.qiagen.com/us/resources/resourcedetail?id=fa2ed17d-a5e8-4843-80c1-3d0ea6c2287d&lang=en.
Biomatrica, DNAgard® Tissue, & Cells Quick Reference Protocol, 2013. http://biomatrica.com/media/dnagard_tissue/9104-0713.pdf.
Qiagen, QIAamp® DNA Investigator Handbook, June 2012. https://www.qiagen.com/gb/resources/download.aspx?id=dcc5a995-3743-4219-914d-94d6a28e49b3&lang=en.
- DNA Typing Methods for Highly Degraded Samples. PhD Thesis. Faculty of Health Sciences and Medicine.Bond University, 2012
ThermoFisher Scientific, AmpFlSTR® Identifiler® Plus PCR Amplification Kit User Guide, 2015. https://tools.thermofisher.com/content/sfs/manuals/cms_076395.pdf.
- Procedures for optimizing the recovery of DNA from mammalian carcasses.Preserv. Genet. Resour. 2015; 7: 333-335
- Systematic study for DNA recovery and profiling from common IED substrates: from laboratory to casework.FSI: Genet. 2015; 17: 53-60