Highlights
- •The NGS results were compared with CE-based STR results in duo cases.
- •MiSeq FGx system could offer comprehensive information of different types of markers.
- •The information from NGS can provide a higher amount of resolution for some samples if there is ambiguity based on CE data.
Abstract
In the case of two mismatches observed in alleged parent-offspring pairs, there is
doubt as to whether there is an exclusion of the putative parent or the existence
of two mutations. Here, we report on four cases with two mismatches in paternal/maternal
duos based on capillary electrophoresis (CE) results. The analyzed next generation
sequencing (NGS) results were compared with 20 autosomal STRs derived from previous
CE-based analysis. In summary, the NGS samples used offered comprehensive information
of different types of markers that can improve resolutions for paternal/maternal duos
analysis.
Keywords
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Article info
Publication history
Published online: May 27, 2016
Accepted:
May 26,
2016
Received in revised form:
May 25,
2016
Received:
December 28,
2015
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
