Highlights
- •We tested the HID-Ion AmpliSeq Ancestry Panel in 104 Greenlanders.
- •Most individuals in a training set were assigned to American or Asian populations.
- •Nine of 15 individuals were assigned to Greenland based on training set.
Abstract
The HID-Ion AmpliSeq Ancestry Panel from Life Techologies includes 123 SNPs from the
Seldin panel and 55 SNPs from Kidd panel in a single multiplex assay that helps to
determine the continental biogeographic ancestry of individuals. We tested the panel
on 104 Greenlanders, divided into a training set of 89 individuals and a test set
of 15 individuals. All loci showed genotype distributions consistent with Hardy-Weinberg
expectations. Linkage disequilibrium tests indicated that 14 pairs of loci were in
association in Greenlanders. Population assignment of the training set to populations
included in the HID SNP genotyper plugin placed most individuals in American, Asian,
and in a few cases European populations. By including the genotype frequencies of
this training set as a possible population of origin, all 15 individuals from the
test set were correctly predicted to be Greenlanders using the Seldin SNPs, and nine
were classified as Greenlanders using the Kidd SNPs. Population structure analysis
indicated that Greenlanders have a genetic profile that is distinguishable from those
of populations from America or Asia.
Keywords
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Article info
Publication history
Published online: June 06, 2016
Accepted:
June 3,
2016
Received in revised form:
May 18,
2016
Received:
January 19,
2016
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
