Several commercial kits are available for Y-chromosome short tandem repeat (STR) haplotyping
for forensic purposes. The PowerPlex® Y23 System (Promega Corp., Madison, MI, USA) was recently released and has been used
worldwide in several populations [
[1]
]. To accumulate South Asian haplotype data, we analyzed 270 DNA samples from Pakistani
Pathans using the PowerPlex® Y23 System, which was previously used to type 22 Y-STRs (17 STRs of AmpFlSTR Yfiler™,
DYS388, DYS446, DYS447, DYS449, and DYS464) [
[2]
]. The full haplotypes for the PowerPlex® Y23 STRs are available on the Y-chromosome haplotype reference database (http://yhrd.org) under the accession number YA003846. While genotyping the 23 Y-STRs, off-ladder
alleles were observed at DYS481 in 20 of the 270 Pathan samples (Table 1). In the meantime, microvariant alleles designated as a one nucleotide intermediate
allele at DYS481 were reported in only 26 of the 19,630 samples from the PowerPlex® Y23 analysis [
[1]
].
Table 1Frequency of the off-ladder alleles at DYS481 observed in 270 Pakistani Pathan samples.
| Allele | Allele | No. of samples | Frequency |
|---|---|---|---|
| by PowerPlex® Y23 | by sequencing | ||
| 18.1 | 18 | 1 | 0.004 |
| 23.1 | 23 | 1 | 0.004 |
| 24.1 | 24 | 7 | 0.026 |
| 25.1 | 25 | 7 | 0.026 |
| 26.1 | 26 | 3 | 0.011 |
| 27.1 | 27 | 1 | 0.004 |
| Total | 20 | 0.074 |
a All samples reported as off-ladder alleles at DYS481 by the PowerPlex® Y23 System contained the L266 mutation.
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References
- A global analysis of Y-chromosomal haplotype diversity for 23 STR loci.Forensic Sci. Int. Genet. 2014; 12: 12-23
- Analysis of 22 Y chromosomal STR haplotypes and Y haplogroup distribution in Pathans of Pakistan.Forensic Sci. Int. Genet. 2014; 11: 111-116
- Haplotype and mutation analysis for newly suggested Y-STRs in Korean father-son pairs.Forensic Sci. Int. Genet. 2015; 15: 64-68
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- Variants observed for STR locus SE33: a concordance study.Forensic Sci. Int. Genet. 2012; 6: 494-497
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Article info
Publication history
Published online: July 01, 2016
Received:
February 22,
2016
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
