Highlights
- •Developmental validation following SWGDAM guidelines of the Yfiler® Plus PCR Amplification Kit.
- •6-dye, 27-plex Y-STR kit that includes the 17 loci from the Yfiler® kit plus 10 new loci including 7 rapidly mutating Y-STRs for improved discrimination of related individuals.
- •Dual application assay designed to amplify DNA from extracted casework samples and database samples from storage cards and swab lysates via direct amplification.
- •Fast time to results with thermal cycling time under 95 min.
- •High concordance between Yfiler® Plus Kit and AmpFlSTR® Yfiler® Kit.
Abstract
Y-chromosomal loci have proven useful in solving investigations where low levels of
male DNA are present in a high female DNA background. An intrinsic limitation of Y-STRs
compared with autosomal STRs is a reduced power of discrimination due to a lack of
recombination throughout most of the Y-chromosome. Thus, in an effort to increase
the power of discrimination we have developed a new 6-dye, 27-plex Y-STR system that
includes the 17 loci from the Yfiler® and Yfiler® Direct kits (DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437,
DYS438, DYS439, DYS448, DYS456, DYS458, DYS635 (Y GATA C4), and Y GATA H4) plus three
highly polymorphic Y-STR loci (DYS460, DYS481, and DYS533), and seven rapidly mutating
Y-STR loci (DYF387S1a/b, DYS449, DYS518, DYS570, DYS576, DYS627) which allow for improved
discrimination of related individuals. The Yfiler® Plus PCR Amplification Kit is a dual application assay designed to amplify DNA from
extracted casework and database samples from storage cards and swab lysates via direct
amplification. Compared to the Yfiler PCR Amplification Kit, the new multiplex shows
increased discrimination of male lineages and also improved performance in inhibited
samples, improved balance in male DNA samples mixed with female DNA at ratios >1:1000,
and faster time to results. The Yfiler Plus Kit shows very high concordance to the
Yfiler Kit but discordance with the PowerPlex® Y23 Kit at the DYS481 locus was observed in 2 out of 30 samples tested. This developmental
validation work follows the SWGDAM guidelines and demonstrates that the assay is robust
and suitable for use on forensic casework and database samples.
Keywords
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Article info
Publication history
Published online: July 08, 2016
Accepted:
July 7,
2016
Received in revised form:
June 23,
2016
Received:
February 18,
2016
Identification
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© 2016 Elsevier Ireland Ltd. All rights reserved.
