Highlights
- •The plasma modified cellulose-chitosan (pCE-CS) membrane was produced as an efficient card to bind, retain and release DNA.
- •DNA recovery from pCE-CS membrane was 5.7 folds greater than the tested commercial card.
- •The obtained DNA had a good quality for PCR amplification.
Abstract
In forensic DNA analyses, biological specimens are collected and stored for subsequent
recovery and analysis of DNA. A cost-effective and efficient DNA recovery approach
is therefore a need. This study aims to produce a plasma modified cellulose-chitosan
membrane (pCE-CS) that efficiently binds and retains DNA as a potential DNA collecting
card. The pCE-CS membrane was produced by a phase separation of ionic liquid dissolving
CE and CS in water with subsequent surface-modification by a two-step exposure of
argon plasma and nitrogen gas. Through plasma modification, the pCE-CS membrane demonstrated
better DNA retention after a washing process and higher rate of DNA recovery as compared
with the original CE-CS membrane and the commercial FTA card. In addition, the pCE-CS
membrane exhibited anti-bacterial properties against both Escherichia coli and Staphylococcus aureus. The results of this work suggest a potential function of the pCE-CS membrane as a
DNA collecting card with a high recovery rate of captured DNA.
Graphical abstract
Graphical Abstract
Keywords
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Article info
Publication history
Published online: July 30, 2016
Accepted:
July 28,
2016
Received in revised form:
June 21,
2016
Received:
March 3,
2016
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
