Highlights
- •A total of 461 individuals from Iraq, Lithuania, Slovenia, and Turkey were typed for 30 InDels using the Investigator® DIPplex Kit.
- •Population and forensic statistical parameters were analyzed and reported.
- •The relative locations of 15 populations in a multidimensional scaling plot drawn from FST distances showed good concordances with their geographic locations.
- •Genotyping issues related to polymorphisms at the primer binding sites for some of the markers in the DIPplex kit are reported.
Abstract
Thirty autosomal insertion-deletion (InDel) polymorphisms were analyzed in four populations
from Iraq, Lithuania, Slovenia, and Turkey using the commercial kit Investigator®
DIPplex. Genotyping issues were encountered for five of the 30 InDels. They were most
probably caused by polymorphisms located in the primer binding sites. Population and
forensic parameters were calculated. No significant deviations from Hardy-Weinberg
equilibrium or significant linkage disequilibrium were detected. The observed heterozygosities
ranged from 33% to 61% depending on the marker and the population. The combined probability
of exclusion for the 30 markers was 99.7% in all four populations and the matching
probabilities were 1 in 3–4 × 1012 individuals. The multidimensional scaling plot drawn from FST distances showed a good concordance between the relative position of the 15 populations
included in the plot and their geographic locations.
Keywords
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References
- A new multiplex for human identification using insertion/deletion polymorphisms.Electrophoresis. 2009; 30: 3682-3690
- A multiplex assay with 52 single nucleotide polymorphisms for human identification.Electrophoresis. 2006; 27: 1713-1724
- Genetic portrait of south Portugal population with InDel markers.Forensic Sci. Int. Genet. 2013; 7: e101-e103
- Forensic performance of two insertion-deletion marker assays.Int. J. Legal Med. 2012; 126: 725-737
- Typing of 30 insertion/deletions in Danes using the first commercial indel kit-mentype (R) DIPplex.Forensic Sci. Int. Genet. 2012; 6: e72-e74
- Population genetic data of 30 autosomal indels in Central Spain and the Basque Country populations.Forensic Sci. Int. Genet. 2013; 7: e27-e30
- Discrimination power of Investigator DIPplex loci in Finnish and Somali populations.Forensic Sci. Int. Genet. 2012; 6: e99-e102
- Population genetics of 30 INDELs in populations of Poland and Taiwan.Mol. Biol. Rep. 2013; 40: 4333-4338
- Population and forensic data for three sets of forensic genetic markers in four ethnic groups from Iran: Persians, Lurs, Kurds and Azeris.Forensic Sci Int Genet. 2015; 17: 43-46
- Results for five sets of forensic genetic markers studied in a Greek population sample.Forensic Sci. Int. Genet. 2015; 16: 132-137
Qiagen, 2014. Investigator DIPplex Handbook, www.qiagen.com/dk/resources/resourcedetail?id=97ae8219-edbf-495b-b6d5-a33b9df6a687&lang=en.
- Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.Biotechniques. 1991; 10: 506-513
- Primer3Plus, an enhanced web interface to Primer3.Nucleic Acids Res. 2007; 35: W71-W74
- Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows.Mol. Ecol. Resour. 2010; 10: 564-567
- A simple sequentially rejective multiple test procedure.Scand. J. Stat. 1979; 6: 65-70
- Selection in finite populations with multiple alleles. 3. Genetic divergence with centripetal selection and mutation.Genetics. 1972; 70: 475-490
- POPTREE2: Software for constructing population trees from allele frequency data and computing other population statistics with Windows interface.Mol. Biol. Evol. 2010; 27: 747-752
- Application of the new insertion-deletion polymorphism kit for forensic identification and parentage testing on the Czech population.Int. J. Legal Med. 2013; 127: 7-10
- A validation study of the Qiagen Investigator DIPplex (R) kit; an INDEL-based assay for human identification.Int. J. Legal Med. 2012; 126: 533-540
- Forensic evaluation of the Investigator DIPplex typing system.Forensic Sci. Int. Genet. 2011; : e331-e332
- A global reference for human genetic variation.Nature. 2015; 526: 68-74
Article info
Publication history
Published online: August 21, 2016
Accepted:
August 19,
2016
Received in revised form:
July 29,
2016
Received:
June 14,
2016
Identification
Copyright
© 2016 Elsevier Ireland Ltd. All rights reserved.
