Highlights
- •A new 10-plex MSRE-PCR system was established to identify the body fluids of venous blood, semen, saliva, vaginal fluids and menstrual blood.
- •The validation of the 10-plex MSRE-PCR system showed that 0.25 ng DNA was sufficient to obtain reproducible identification of biofluid types.
- •The MSRE-PCR system and AmpFlSTR® MiniFiler™ were co-amplified in a single reaction to generate both biofluid and miniSTR profiles.
Abstract
The identification of a specific body fluid encountered in a forensic investigation
can give crucial information. This identification can be aided by methylation profiles
based on selected markers specific to a range of biofluids. In this study, the open
database of Infinium HumanMethylation450 BeadChip was searched for markers specific
for semen, vaginal fluids, saliva, venous blood and menstrual blood. A total of 8
biofluid-specific methylated markers and 2 control markers were combined into a 10-plex
methylation sensitive restriction enzyme-PCR (MSRE-PCR) system. Based upon the analysis
of 100 DNA samples from these 5 biofluid types, unambiguous results were obtained
to identify the body fluid from which it originated. Validation studies of the developed
10-plex MSRE-PCR included sensitivity, reproducibility and mixed body fluids. Co-amplification
of the established MSRE-PCR system and the microsatellite loci in AmpFlSTR® MiniFiler™
PCR Amplification Kit was performed to generate both the methylation profile for biofluid
type and the miniSTR profile. This allowed human identification and the identification
of the body fluid type to be performed in a single reaction. The results of this study
displayed the applicability of this 10-plex MSRE-PCR system in forensic science.
Graphical abstract
Graphical Abstract
Keywords
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Article info
Publication history
Published online: September 02, 2016
Accepted:
August 31,
2016
Received in revised form:
July 28,
2016
Received:
May 4,
2016
Identification
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© 2016 Elsevier Ireland Ltd. All rights reserved.
