Highlights
- •A population analysis was performed on 10 urban populations of Argentina for a set of 12 autosomal STR not studied to date in the country.
- •Population comparisons were carried out observing a relative homogenous allelic frequency distribution across the country with the exception of Jujuy population.
- •Enhanced Power of Discrimination and Power of Exclusion was observed for this marker set compared the 13 STR CODIS loci.
- •The system appeared to be a useful tool to be used as a complement in complex cases.
Abstract
Currently, autosomal Short Tandem Repeat (STR) markers represent the method of election
in forensic human identification. Commercial kits of most common use nowadays –e.g.
PowerPlex®Fusion, Promega Corp.; AmpFlSTR GlobalFiler, Thermofisher scientific; Investigator
24Plex QS,Qiagen-, allow the co-amplification of 23 highly polymorphic STR loci providing
a high discrimination power in human identity testing. However, in complex kinship
analysis and familial database searches involving distant relationships, additional
DNA typing is often required in order to achieve well-founded conclusions. The recently
developed kit Investigator® HDplex (Qiagen) co-amplify twelve autosomal STRs markers (D7S1517, D3S1744, D12S391,
D2S1360, D6S474, D4S2366, D8S1132, D5S2500, D18S51, D21S2055, D10S2325, SE33), nine
of which are not present in the above mentioned kits, providing a set of efficient
supplementary markers for human identification purposes. In this study we genotyped
a sample of 980 individuals from urban areas of ten Argentinean provinces using the
Investigator® HDplex kit, aiming to provide forensic estimates for use in forensic casework and
parentage testing in Argentina. We report reference allelic frequency databases for
each of the provinces studied as well as for the combined samples. No deviation of
Hardy-Weinberg equilibrium was observed. A reasonable discrimination capacity and
power of exclusion was estimated which allowed predicting an acceptable forensic behavior
of this kit, either to be used as the main STR panel for simple cases or as an auxiliary
tool in complex cases. Additionally, population comparison tests showed that the studied
samples are relatively homogeneous across the country for these STR set.
Keywords
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Article info
Publication history
Published online: October 18, 2016
Accepted:
October 17,
2016
Received in revised form:
September 13,
2016
Received:
June 8,
2016
Identification
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© 2016 Elsevier Ireland Ltd. All rights reserved.
