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Correspondence| Volume 26, e25-e27, January 2017

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Validation studies on dinucleotide STRs for forensic identification of black rhinoceros Diceros bicornis

Published:October 24, 2016DOI:https://doi.org/10.1016/j.fsigen.2016.10.016
      Rhinoceros populations are, for the second time in 50 years, undergoing a poaching crisis driven by the illegal trade in rhinoceros horn for use in traditional Asian medicine [

      T. Milliken J. Shaw, The South Africa—Viet Nam Rhino Horn Trade Nexus: A deadly combination of institutional lapses, corrupt wildlife industry professionals and Asian crime syndicates, TRAFFIC, Johannesburg, South Africa (2012).

      ]. By 2013, on average three rhinoceros were illegally killed per day in South Africa [

      T. Milliken, Illegal Trade in Ivory and Rhino Horn: an Assessment Report to Improve Law Enforcement Under the Wildlife TRAPS Project, TRAFFIC, Cambridge, UK, https://www.usaid.gov/sites/default/files/documents/1865/W-TRAPS-Elephant-Rhino-report.pdf (accessed 08.07.16) (2014).

      ]. Furthermore, horns have entered the illegal trade from other sources, including thefts from museums, private collections and government stockpiles [

      T. Milliken J. Shaw, The South Africa—Viet Nam Rhino Horn Trade Nexus: A deadly combination of institutional lapses, corrupt wildlife industry professionals and Asian crime syndicates, TRAFFIC, Johannesburg, South Africa (2012).

      ,

      R.H. Emslie, T. Milliken, B. Talukdar, African and Asian Rhinoceroses—Status, Conservation and Trade, CoP16 Doc. 54.2 (Rev. 1) Annex 2, CITES Secretariat, Geneva, Switzerland (2012).

      ]. The application of DNA techniques to assist law enforcement by tracing horn recovered in illegal trade is crucial [
      • Harper C.K.
      • Vermeulen G.J.
      • Clarke A.B.
      • De Wet J.I.
      • Guthrie A.J.
      Extraction of nuclear DNA from rhinoceros horn and characterization of DNA profiling systems for white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros.
      ], and yet the forensic validation of STR markers in endangered species is problematic for many reasons.

      Keywords

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      References

      1. T. Milliken J. Shaw, The South Africa—Viet Nam Rhino Horn Trade Nexus: A deadly combination of institutional lapses, corrupt wildlife industry professionals and Asian crime syndicates, TRAFFIC, Johannesburg, South Africa (2012).

      2. T. Milliken, Illegal Trade in Ivory and Rhino Horn: an Assessment Report to Improve Law Enforcement Under the Wildlife TRAPS Project, TRAFFIC, Cambridge, UK, https://www.usaid.gov/sites/default/files/documents/1865/W-TRAPS-Elephant-Rhino-report.pdf (accessed 08.07.16) (2014).

      3. R.H. Emslie, T. Milliken, B. Talukdar, African and Asian Rhinoceroses—Status, Conservation and Trade, CoP16 Doc. 54.2 (Rev. 1) Annex 2, CITES Secretariat, Geneva, Switzerland (2012).

        • Harper C.K.
        • Vermeulen G.J.
        • Clarke A.B.
        • De Wet J.I.
        • Guthrie A.J.
        Extraction of nuclear DNA from rhinoceros horn and characterization of DNA profiling systems for white (Ceratotherium simum) and black (Diceros bicornis) rhinoceros.
        Forensic Sci. Int. Genet. 2013; 7: 428-433https://doi.org/10.1016/j.fsigen.2013.04.003
        • Linacre A.
        • Gusmão L.
        • Hecht W.
        • Hellmann A.P.
        • Mayr W.R.
        • Parson W.
        • Prinz M.
        • Schneider P.
        • Morling N.
        ISFG: recommendations regarding the use of non-human (animal) DNA in forensic genetic investigations.
        Forensic Sci. Int. Genet. 2011; 5: 501-505https://doi.org/10.1016/j.fsigen.2010.10.017
        • Scott C.
        Microsatellite Variability in Four Contemporary Rhinocerous Species: Implications for Conservation.
        Queen’s University, 2008
        • du Toit R.
        African rhino systematics—the existing basis for subspecies classification of black and white rhinos.
        Pachyderm. 1987; 9: 1-2
        • Muya S.M.
        • Bruford M.W.
        • Muigai A.W.T.
        • Osiemo Z.B.
        • Mwachiro E.
        • Okita-Ouma B.
        • Goossens B.
        Substantial molecular variation and low genetic structure in Kenya’s black rhinoceros: implications for conservation.
        Conserv. Genet. 2011; 12: 1575-1588https://doi.org/10.1007/s10592-011-0256-3
        • Van Oosterhout C.
        • Hutchinson W.F.
        • Wills D.P.M.
        • Shipley P.
        MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data.
        Mol. Ecol. Notes. 2004; 4: 535-538https://doi.org/10.1111/j.1471-8286.2004.00684.x
        • Peakall R.
        • Smouse P.E.
        GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research.
        Mol. Ecol. Notes. 2006; 6: 288-295https://doi.org/10.1111/j.1471-8286.2005.01155.x
        • Peakall R.
        • Smouse P.E.
        GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research—an update.
        Bioinformatics. 2012; 28: 2537-2539https://doi.org/10.1093/bioinformatics/bts460
        • Weir B.S.
        Statistics for wildlife forensic DNA.
        in: Huffman J.E. Wallace J.R. Wildl. Forensics Methods Appl. Wiley-Blackwell, Chichester2012: 237-252https://doi.org/10.1002/9781119953142.ch12