Highlights
- •We constructed two multiplex PCR sets for the amplification of 13 RM Y-STR.
- •Four copies of DYF403S1 could be separately amplified into three subtypes.
- •More diverse haplotypes were obtained by redefining of DYF403S1.
- •The haplotypes for 37 Y-STRs were examined in 705 unrelated Korean males.
- •Every individual of 705 unrelated Korean males could be discriminated by 13 RM Y-STRs.
Abstract
In forensic science and human genetics, Y-chromosomal short tandem repeats (Y-STRs)
have been used as very useful markers. Recently, more Y-STR markers have been analyzed
to enhance the resolution power in haplotype analysis, and 13 rapidly mutating (RM)
Y-STRs have been suggested as revolutionary tools that can widen Y-chromosomal application
from paternal lineage differentiation to male individualization. We have constructed
two multiplex PCR sets for the amplification of 13 RM Y-STRs, which yield small-sized
amplicons (<400 bp) and a more balanced PCR efficiency with minimum PCR cycling. In particular, with
the developed multiplex PCR system, we could separate three copies of DYF403S1a into
two copies of DYF403S1a and one of DYF403S1b1. This is because DYF403S1b1 possesses
distinguishable sequences from DYF403S1a at both the front and rear flanking regions
of the repeat motif; therefore, the locus could be separately amplified using sequence-specific
primers. In addition, the other copy, defined as DYF403S1b by Ballantyne et al., was
renamed DYF403S1b2 because of its similar flanking region sequence to DYF403S1b1.
By redefining DYF403S1 with the developed multiplex system, all genotypes of four
copies could be successfully typed and more diverse haplotypes were obtained. We analyzed
haplotype distributions in 705 Korean males based on four different Y-STR subsets:
Yfiler, PowerPlex Y23, Yfiler Plus, and RM Y-STRs. All haplotypes obtained from RM
Y-STRs were the most diverse and showed strong discriminatory power in Korean population.
Keywords
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Article info
Publication history
Published online: October 27, 2016
Accepted:
October 26,
2016
Received in revised form:
September 28,
2016
Received:
April 15,
2016
Identification
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© 2016 Elsevier Ireland Ltd. All rights reserved.
