Advertisement

A selection guide for the new generation 6-dye DNA profiling systems

      Highlights

      • The performance of three new STR profiling systems with 6-dye chemistry is tested.
      • GlobalFiler displays the highest sensitivity.
      • Investigator 24plex shows a higher tolerance to common PCR inhibitors.
      • GlobalFiler & Fusion 6C yields profiles with better PHR & intra-colour balance.
      • Fusion 6C displays a higher consistency in the preservation of the mixture ratio.

      Abstract

      The Federal Bureau of Investigation (FBI) has recently expanded the CODIS core loci from the existing 13 to 20 as a new guideline, and laboratories from the US are required to comply with the new regulation before uploading or conducting identity search in the national database. The expanded CODIS format, which shares all the core loci commonly used in the European countries and the US, not only increases international compatibility, but also reduces the number of adventitious matches, and hence facilitates international law enforcement and counterterrorism endeavours. Here, we review the key performance measures of three new STR amplification systems with 6-dye chemistry, namely, the Investigator 24plex QS Kit from QIAGEN, the GlobalFiler™ PCR Amplification Kit from Applied Biosystems™, and the PowerPlex® Fusion 6C System from Promega. Our results have demonstrated that GlobalFiler displays the highest sensitivity among the tested kits, whereas Investigator 24plex shows a higher tolerance to common PCR inhibitors including Humic acid and Tannic acid. GlobalFiler and Fusion 6C, on the other hand, yield DNA profiles with better heterozygous peak height and intra-colour signal balance. Both GlobalFiler and Investigator 24plex exhibit slightly higher sensitivity than Fusion 6C in the profiling of minor components in DNA mixture, but the latter displays a higher consistency in the preservation of the mixture ratio. In summary, our work has demonstrated that these three profiling systems have their different performance features, and hence it is recommended that laboratories should select the most suitable kits according to their own requirements and operational needs.

      Keywords

      To read this article in full you will need to make a payment

      Purchase one-time access:

      Academic & Personal: 24 hour online accessCorporate R&D Professionals: 24 hour online access
      One-time access price info
      • For academic or personal research use, select 'Academic and Personal'
      • For corporate R&D use, select 'Corporate R&D Professionals'

      Subscribe:

      Subscribe to Forensic Science International: Genetics
      Already a print subscriber? Claim online access
      Already an online subscriber? Sign in
      Institutional Access: Sign in to ScienceDirect

      References

        • Gill P.
        • Jeffreys A.J.
        • Werrett D.J.
        Forensic application of DNA ‘fingerprints’.
        Nature. 1985; 318: 577-579
        • Jeffreys A.J.
        • Wilson V.
        • Thein S.L.
        Individual-specific ‘fingerprints’ of human DNA.
        Nature. 1985; 316: 76-79
        • Hares D.R.
        Addendum to expanding the CODIS core loci in the United States.
        Forensic Sci. Int. Genet. 2012; 6: e135
        • Hares D.R.
        Expanding the CODIS core loci in the United States.
        Forensic Sci. Int. Genet. 2012; 6: e52-4
        • Hares D.R.
        Selection and implementation of expanded CODIS core loci in the United States.
        Forensic Sci. Int. Genet. 2015; 17: 33-34
        • Scherer M.
        • König M.
        • Breitbach M.
        • Cornelius S.
        • Bussmann M.
        • Prochnow A.
        • et al.
        Improving sample analysis and interpretation using QIAGEN's latest Investigator STR multiplex PCR assays with a novel quality sensor.
        Forensic Sci. Int. Genet. Suppl. 2015; 5: 308-309
      1. Investigator® 24plex QS Handbook (November 2014).

        • Kraemer M.
        • Prochnow A.
        • Bussmann M.
        • Scherer M.
        • Peist R.
        • Steffen C.
        Developmental validation of QIAGEN Investigator® 24plex QS kit and Investigator® 24plex GO! kit: two 6-dye multiplex assays for the extended CODIS core loci.
        Forensic Sci. Int. Genet. 2017; 29: 9-20
      2. GlobalFiler™ PCR Amplification Kit User Guide (2015).

        • Martin P.
        • de Simon L.F.
        • Luque G.
        • Farfan M.J.
        • Alonso A.
        Improving DNA data exchange: validation studies on a single 6 dye STR kit with 24 loci.
        Forensic Sci. Int. Genet. 2014; 13: 68-78
        • Bogas V.
        • Carvalho M.
        • Corte-Real F.
        • Porto M.J.
        Testing the behavior of GlobalFiler PCR amplification kit with degraded and/or inhibited biological samples.
        Forensic Sci. Int. Genet. Suppl. 2015; 5: 21-23
        • Ensenberger M.G.
        • Lenz K.A.
        • Matthies L.K.
        • Hadinoto G.M.
        • Schienman J.E.
        • Przech A.J.
        • et al.
        Developmental validation of the PowerPlex® fusion 6C system.
        Forensic Sci. Int. Genet. 2016; 21: 134-144
      3. PowerPlex® Fusion 6C System Technical Manual (10/15).

        • Cisana S.
        • Cerri N.
        • Bosetti A.
        • Verzeletti A.
        • Cortellini V.
        PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples.
        Croat. Med. J. 2017; 58: 26-33
      4. AmpFlSTR® Identifiler® Plus PCR Amplification Kit User Guide (2015).

        • Wang D.Y.
        • Chang C.W.
        • Lagace R.E.
        • Calandro L.M.
        • Hennessy L.K.
        Developmental validation of the AmpFlSTR® Identifiler® Plus PCR Amplification Kit: an established multiplex assay with improved performance.
        J. Forensic Sci. 2012; 57: 453-465
        • Walsh P.S.
        • Metzger D.A.
        • Higuchi R.
        Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material.
        Biotechniques. 1991; 10: 506-513
        • Cláudia V.S.
        • Heloísa A.C.
        • Ribeiro T.
        • Porto M.J.
        • Dias M.
        • Amorim A.
        Quantifiler Trio DNA validation and usefulness in casework samples.
        Forensic Sci. Int. Genet. Suppl. 2015; 5: 246-247
      5. Quantifiler® HP and Trio DNA Quantification Kits User Guide (2014).

        • Gouveia N.
        • Brito P.
        • Serra A.
        • Balsa F.
        • Andrade L.
        • São Bento M.
        • et al.
        Validation of quantifiler trio DNA quantification kit in forensic samples.
        Forensic Sci. Int. Genet. Suppl. 2015; 5: 24-25
        • Pflugbeil A.M.
        • Groß S.
        • Rothe J.
        • Heinke F.
        • Bruchhaus H.
        • Edelmann J.
        • et al.
        Initial experience in the application of the PowerQuant system and the Investigator ESSplex SE QS kit for aDNA analysis.
        Forensic Sci. Int. Genet. Suppl. 2015; 5: 420-421
      6. PowerQuant™ System Technical Manual (3/15).