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A forensic population database in El Salvador: 58 STRs and 94 SNPs

  • Ferran Casals
    Affiliations
    Genomics Core Facility, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Catalonia, Spain

    Departament de Genètica, Microbiologia i Estadísitca, Universitat de Barcelona, Barcelona, Spain
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  • Raquel Rasal
    Affiliations
    Genomics Core Facility, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Catalonia, Spain
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  • Roger Anglada
    Affiliations
    Genomics Core Facility, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Catalonia, Spain
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  • Marc Tormo
    Affiliations
    Genomics Core Facility, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Catalonia, Spain

    Scientific IT Core Facility, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Catalonia, Spain
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  • Núria Bonet
    Affiliations
    Genomics Core Facility, Departament de Ciències Experimentals i de la Salut, Universitat Pompeu Fabra, Parc de Recerca Biomèdica de Barcelona, 08003 Barcelona, Catalonia, Spain
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  • Nury Rivas
    Affiliations
    Instituto de Medicina Legal Dr. Roberto Masferrer, San Salvador, El Salvador
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  • Patricia Vásquez
    Correspondence
    Corresponding authors.
    Affiliations
    Asociación Pro-Búsqueda de Niñas y Niños Desaparecidos de El Salvador, 27 calle Pnte. No.1329 Colonia Layco, San Salvador, El Salvador
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  • Francesc Calafell
    Correspondence
    Corresponding authors.
    Affiliations
    Institute of Evolutionary Biology (UPF-CSIC), Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain
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Published:December 01, 2021DOI:https://doi.org/10.1016/j.fsigen.2021.102646

      Highlights

      • Genotyped 58 STRs (27 autosomal, 24 Y-STRs and 7 X-STRs) and 94 autosomal SNPs.
      • Samples from the general population of El Salvador (ES, Central America).
      • Used Verogen ForenSeq™ Primer Mix A to generate length and repeat-sequence alleles.
      • Extremely low overall RMP means it can be applied successfully even if many loci fail.
      • Could be used to identify missing people from ES civil war and deceased migrants.

      Abstract

      We have genotyped the 58 STRs (27 autosomal, 24 Y-STRs and 7 X-STRs) and 94 autosomal SNPs in Illumina ForenSeq™ Primer Mix A in a sample of 248 men and 143 women from El Salvador, Central America. Regional division (Centro, Oriente, Occidente) showed in almost all cases FST values not significantly different from 0, and further analyses were applied only to the undivided, country-wide population. The overall random match probability (RMP) decreased from 6.79 × 10−31 in length-based genotypes in the 27 autosomal STRs to 1.47 × 10−34 in repeat-sequence based genotypes. Combining the autosomal loci in this set, RMP reaches 2.97 × 10−70. In a population genetic analysis, El Salvador showed the lowest FST values with US Hispanics both for autosomal and X-STRs; however, it was much closer to Native Americans for the latter than for the former, in accordance with the well-known gender-biased admixture that created most Latin American populations.

      Keywords

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