Highlights
- •The ForenSeq Signature Kit was used to sequence 243 individuals from 17 families.
- •Both the LR and IBS methods were used for different kinship analysis.
- •Different markers/marker combinations were used in LR and IBS calculation.
Abstract
With the tremendous development of massively parallel sequencing (MPS) in the last
decade, it has been widely applied in basic science, clinical diagnostics, microbial
genomics, as well as forensic genetics. MPS has lots of advantages that may facilitate
the kinship analysis. In this study, 243 Chinese Han individuals from 17 families
were involved and sequenced using the ForenSeq™ DNA Signature Prep Kit (Verogen, Inc.,
San Diego, USA), which provided the sequence information of 27 autosomal STRs (A-STRs),
7 X chromosomal STRs (X-STRs), 24 Y chromosomal STRs (Y-STRs) and 94 identity-informative
SNPs (iSNPs). A total of 275 pairs of parent-child, 123 pairs of full siblings, 1
pair of twins, 1 pair of half siblings, 158 pairs of grandparent-grandchild, 222 pairs
of uncle/aunt-nephew/niece and 121 pairs of first cousins, as well as 701 pairs of
unrelated individuals were identified. Using both likelihood ratio (LR) and identical
by state (IBS) methods, the kinship analysis was conducted among these relative and
non-relative pairs based on the A-STRs and SNPs. As a result, the ForenSeq Signature
Kit could solve the analysis of parent-child (t1 = −4, t2 = 4), full siblings (t1 = −2,
t2 = 2) and most second-degree kinships (t1 = −1, t2 = 1) using the LR method. When
the IBS method was applied, 123 full sibling pairs had a higher average IBS value
than other kinship groups in this study. And the IBS method could play a role in the
testing of parent-child and full siblings.
Keywords
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Article info
Publication history
Published online: November 30, 2021
Accepted:
November 28,
2021
Received in revised form:
November 18,
2021
Received:
July 12,
2021
Identification
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