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Case report| Volume 59, 102718, July 2022

Genetic and isotope analysis of a triple burial from medieval St. Peter’s cemetery in Cölln/Berlin

      Highlights

      • triple burial from medieval Berlin/Cölln reveals remains of three men in a crouching position and extensive injuries.
      • genetic analysis indicates no close biological kinship between the individuals.
      • genetic and isotope analyses of geographical origin assignes two individuals as local and one with a possible distant origin.
      • data suggests that the shared grave arose from special circumstances of their death and the necessity of transport of the corpses to Berlin/Cölln.

      Abstract

      The German capital Berlin originates from the two medieval settlements Berlin and Cölln on either side of the river Spree. Whereas Berlin is world famous, there is very little awareness of former Cölln. From 2007–2009, during excavations of the earliest cemetery of this forgotten medieval town; 3,126 graves were discovered containing the remains of 3,717 individuals. Amongst those graves was an unusual triple burial. This grave was exceptional due to the relative postures of the skeletons and their extensive facial injuries. Here we present genetic and isotope data for this grave. Genetic results confirmed all of them as biological male individuals and ruled out their biological kinship. Combining genetic ancestry information with strontium isotope data we furthermore determined that two of the men most likely originated from the Berlin-Brandenburg region, whereas a more distant origin of the third individual can be debated.

      Keywords

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      References

        • Melisch C.M.
        Die Ausgrabungen am Petriplatz.
        Ergebnisse der archäologischen Untersuchung, Der Petriplatz in Berlin-Mitte - Archäologisch-historische Studien. Hendrik Bäßler Verlag Berlin, Berlin2020: 17-93
      1. C.M. Melisch, J. Sewell, Omnia mors aequat and other problems with processing large data from a cemetery in: A.J. Nijboer, S.L. Willemsen, P.A.J. Attema, J.F. Seubers (Eds.), Research Into Pre-Roman Burial Grounds in Italy, CAECVLVS. Papers in Mediterranean Archaeology and Greek and Roman Studies 2014, pp. 169–183.

      2. C.M. Melisch, P. Rauxloh, N. Powers, I. Garlisch, Medieval Space and Population, in: W. Börner, S. Uhlitz (Eds.) International Conference on Cultural Heritage and New Technologies, Vienna, 2014.

        • Melisch C.M.
        • Radetskyi K.
        • Knaut M.
        Synchron ausgraben und forschen - Petriplatz Berlin, HTW Berlin, Matthias Knaut, Kreativwirtschaft.
        Beiträge und Positionen. BWV Berliner Wissenschafts-Verlag, Berlin2011: 238-243
        • Powers N.
        Methoden und Ablauf.
        Der Petriplatz in Berlin-Mitte - Archäologisch-historische Studien. hendrik Bäßler verlag Berlin, 2020: 175-178
        • Melisch C.M.
        • Garlisch I.
        • Jungklaus B.
        • Killgrove K.
        • Nagy M.
        • Powers N.
        • Rothe J.
        • Teßmann B.
        • Tichomirowa M.
        • White K.
        Auf der Suche nach den ersten Berlinern. Das internationale Forschungsprojekt „Medieval Space and Population“.
        Mitteilungen der Berliner Gesellschaft für Anthropologie, Ethnologie und Urgeschichte. Vol. 37. Verlag Marie Leidorf GmbH,, 2016: 51-64
      3. N. Powers, Das Kistengrab 3443, Der Petriplatz in Berlin-Mitte - Archäologisch-historische Studien, hendrik Bäßler verlag Berlin, Berlin, 2020a, pp. 204–214.

        • Kromer B.
        • Lindauer S.
        • Synal H.-A.
        • Wacker L.
        MAMS - a new AMS facility at the Curt-Engelhorn-Centre for Achaeometry, Mannheim, Germany.
        Nucl. Instrum. Methods Phys. Res. Sect. B: Beam Interact. Mater. At. 2013; 294: 11-13
        • Brown T.A.
        • Nelson D.E.
        • Vogel J.S.
        • Southon J.R.
        Improved collagen extraction by modified Longin method.
        Radiocarbon. 1988; 30: 171-177
        • Reimer P.J.
        • Austin W.E.N.
        • Bard E.
        • Bayliss A.
        • Blackwell P.G.
        • Bronk Ramsey C.
        • Butzin M.
        • Cheng H.
        • Edwards R.L.
        • Friedrich M.
        • Grootes P.M.
        • Guilderson T.P.
        • Hajdas I.
        • Heaton T.J.
        • Hogg A.G.
        • Hughen K.A.
        • Kromer B.
        • Manning S.W.
        • Muscheler R.
        • Palmer J.G.
        • Pearson C.
        • van der Plicht J.
        • Reimer R.W.
        • Richards D.A.
        • Scott E.M.
        • Southon J.R.
        • Turney C.S.M.
        • Wacker L.
        • Adolphi F.
        • Büntgen U.
        • Capano M.
        • Fahrni S.M.
        • Fogtmann-Schulz A.
        • Friedrich R.
        • Köhler P.
        • Kudsk S.
        • Miyake F.
        • Olsen J.
        • Reinig F.
        • Sakamoto M.
        • Sookdeo A.
        • Talamo S.
        The IntCal20 northern hemisphere radiocarbon age calibration curve (0–55 cal kBP).
        Radiocarbon. 2020; 62: 725-757
        • Bronk Ramsey C.
        Radiocarbon calibration and analysis of stratigraphy: the OxCal program.
        Radiocarbon. 1995; 37: 425-430
        • Barta P.
        • Štolc Jr., S.
        HBCO correction: its impact on archaeological absolute dating.
        Radiocarbon. 2007; 49: 465-472
        • Schuchard C.
        • Gründungsurkunde Keine
        Symeon plebanus de Colonia als Zeuge - Die erste urkundliche Erwähnung von Cölln an der Spree 1237/38.
        Berl. Gesch. und Ggw. Jahrb. Des. Landesarch. Berl. 1987; : 7-36
        • Zechini M.E.
        • Killgrove K.
        • Melisch C.M.
        • Turner B.L.
        • Schaefer B.J.
        Diachronic changes in diet in medieval Berlin: Comparison of dietary isotopes from pre- and post-Black Death adults.
        J. Archaeol. Sci.: Rep. 2021; 38103064
        • Rothe J.
        • Melisch C.
        • Powers N.
        • Geppert M.
        • Zander J.
        • Purps J.
        • Spors B.
        • Nagy M.
        Genetic research at a fivefold children's burial from medieval Berlin.
        Forensic Sci. Int. Genet. 2015; 15: 90-97
        • Willuweit S.
        • Roewer L.
        • Y.C.U.G. International Forensic
        Y chromosome haplotype reference database (YHRD): update.
        Forensic Sci. Int. Genet. 2007; 1: 83-87
        • Nunez C.
        • Geppert M.
        • Baeta M.
        • Roewer L.
        • Martinez-Jarreta B.
        Y chromosome haplogroup diversity in a Mestizo population of Nicaragua.
        Forensic Sci. Int. Genet. 2012; 6: e192-e195
        • Haas C.
        • Shved N.
        • Ruhli F.J.
        • Papageorgopoulou C.
        • Purps J.
        • Geppert M.
        • Willuweit S.
        • Roewer L.
        • Krawczak M.
        Y-chromosomal analysis identifies the skeletal remains of Swiss national hero Jorg Jenatsch (1596-1639).
        Forensic Sci. Int. Genet. 2013; 7: 610-617
        • Eichmann C.
        • Parson W.
        ‘Mitominis’: multiplex PCR analysis of reduced size amplicons for compound sequence analysis of the entire mtDNA control region in highly degraded samples.
        Int. J. Leg. Med. 2008; 122: 385-388
        • Parson W.
        • Gusmao L.
        • Hares D.R.
        • Irwin J.A.
        • Mayr W.R.
        • Morling N.
        • Pokorak E.
        • Prinz M.
        • Salas A.
        • Schneider P.M.
        • Parsons T.J.
        • D.N.A.C.o.t.I.S.f.F. Genetics
        DNA commission of the International Society for Forensic Genetics: Revised and extended guidelines for mitochondrial DNA typing.
        Forensic Sci. Int. Genet. 2014; 13: 134-142
        • van Oven M.
        • Kayser M.
        Updated comprehensive phylogenetic tree of global human mitochondrial DNA variation.
        Hum. Mutat. 2009; 30: E386-E394
        • Walsh S.
        • Chaitanya L.
        • Clarisse L.
        • Wirken L.
        • Draus-Barini J.
        • Kovatsi L.
        • Maeda H.
        • Ishikawa T.
        • Sijen T.
        • de Knijff P.
        • Branicki W.
        • Liu F.
        • Kayser M.
        Developmental validation of the HIrisPlex system: DNA-based eye and hair colour prediction for forensic and anthropological usage.
        Forensic Sci. Int. Genet. 2014; 9: 150-161
        • Chaitanya L.
        • Breslin K.
        • Zuniga S.
        • Wirken L.
        • Pospiech E.
        • Kukla-Bartoszek M.
        • Sijen T.
        • Knijff P.
        • Liu F.
        • Branicki W.
        • Kayser M.
        • Walsh S.
        The HIrisPlex-S system for eye, hair and skin colour prediction from DNA: Introduction and forensic developmental validation.
        Forensic Sci. Int. Genet. 2018; 35: 123-135
        • Bodner M.
        • Bastisch I.
        • Butler J.M.
        • Fimmers R.
        • Gill P.
        • Gusmao L.
        • Morling N.
        • Phillips C.
        • Prinz M.
        • Schneider P.M.
        • Parson W.
        Recommendations of the DNA Commission of the International Society for Forensic Genetics (ISFG) on quality control of autosomal short tandem repeat allele frequency databasing (STRidER).
        Forensic Sci. Int. Genet. 2016; 24: 97-102
        • Charlier B.
        • Ginibre C.
        • Morgan D.
        • Nowell G.
        • Pearson G.
        • Davidson J.
        • Ottley C.
        Methods for the microsampling and high-precision analysis of strontium and rubidium isotopes at single crystal scale for petrological and geochronological applications.
        Chem. Geol. 2006; 232: 114-133
        • Thirlwall M.F.
        Long-term reproducibility of multicollector Sr and Nd isotope ratio analysis.
        Chem. Geol. 1991; 94: 85-104
        • Price T.D.
        • Burton J.H.
        • Bentley R.A.
        The characterization of biologically available strontium isotope ratios for the study of prehistoric migration.
        Archaeometry. 2002; 44: 117-135
        • Hillson S.
        Teeth. second ed. Cambridge University Press, Cambridge2005
        • Voerkelius S.
        • Lorenz G.
        • Rummel S.
        • Quétel C.
        • Heiss G.
        • Baxter M.
        • Brach-Papa C.
        • Deters-Itzelsberger P.
        • Hoelzl S.
        • Hoogewerff J.
        • Ponzevera E.
        • Bocxstaele M.
        • Ueckermann H.
        Strontium isotopic signatures of natural mineral waters, the reference to a simple geological map and its potential for authentication of food.
        Food Chem. 2010; 118: 933-940
        • Regenspurg S.
        • Feldbusch E.
        • Norden B.
        • Tichomirowa M.
        Fluid-rock interactions in a geothermal Rotliegend/ Permo-Carboniferous reservoir (North German Basin).
        Appl. Geochem. 2016; 69
        • Jobling M.A.
        • Hurles M.E.
        • Tyler-Smith C.
        Human Evolutionary Genetics: Origins, Peoples and Disease.
        Garland Science, London2004
        • Myres N.M.
        • Rootsi S.
        • Lin A.A.
        • Jarve M.
        • King R.J.
        • Kutuev I.
        • Cabrera V.M.
        • Khusnutdinova E.K.
        • Pshenichnov A.
        • Yunusbayev B.
        • Balanovsky O.
        • Balanovska E.
        • Rudan P.
        • Baldovic M.
        • Herrera R.J.
        • Chiaroni J.
        • Di Cristofaro J.
        • Villems R.
        • Kivisild T.
        • Underhill P.A.
        A major Y-chromosome haplogroup R1b Holocene era founder effect in Central and Western Europe.
        Eur. J. Hum. Genet. 2011; 19: 95-101
        • Rootsi S.
        • Magri C.
        • Kivisild T.
        • Benuzzi G.
        • Help H.
        • Bermisheva M.
        • Kutuev I.
        • Barac L.
        • Pericic M.
        • Balanovsky O.
        • Pshenichnov A.
        • Dion D.
        • Grobei M.
        • Zhivotovsky L.A.
        • Battaglia V.
        • Achilli A.
        • Al-Zahery N.
        • Parik J.
        • King R.
        • Cinnioglu C.
        • Khusnutdinova E.
        • Rudan P.
        • Balanovska E.
        • Scheffrahn W.
        • Simonescu M.
        • Brehm A.
        • Goncalves R.
        • Rosa A.
        • Moisan J.P.
        • Chaventre A.
        • Ferak V.
        • Furedi S.
        • Oefner P.J.
        • Shen P.
        • Beckman L.
        • Mikerezi I.
        • Terzic R.
        • Primorac D.
        • Cambon-Thomsen A.
        • Krumina A.
        • Torroni A.
        • Underhill P.A.
        • Santachiara-Benerecetti A.S.
        • Villems R.
        • Semino O.
        Phylogeography of Y-chromosome haplogroup I reveals distinct domains of prehistoric gene flow in europe.
        Am. J. Hum. Genet. 2004; 75: 128-137
        • Cruciani F.
        • La Fratta R.
        • Trombetta B.
        • Santolamazza P.
        • Sellitto D.
        • Colomb E.B.
        • Dugoujon J.M.
        • Crivellaro F.
        • Benincasa T.
        • Pascone R.
        • Moral P.
        • Watson E.
        • Melegh B.
        • Barbujani G.
        • Fuselli S.
        • Vona G.
        • Zagradisnik B.
        • Assum G.
        • Brdicka R.
        • Kozlov A.I.
        • Efremov G.D.
        • Coppa A.
        • Novelletto A.
        • Scozzari R.
        Tracing past human male movements in northern/eastern Africa and western Eurasia: new clues from Y-chromosomal haplogroups E-M78 and J-M12.
        Mol. Biol. Evol. 2007; 24: 1300-1311
        • Richards M.
        • Corte-Real H.
        • Forster P.
        • Macaulay V.
        • Wilkinson-Herbots H.
        • Demaine A.
        • Papiha S.
        • Hedges R.
        • Bandelt H.J.
        • Sykes B.
        Paleolithic and neolithic lineages in the European mitochondrial gene pool.
        Am. J. Hum. Genet. 1996; 59: 185-203
        • Richards M.
        • Macaulay V.
        • Torroni A.
        • Bandelt H.J.
        In search of geographical patterns in European mitochondrial DNA.
        Am. J. Hum. Genet. 2002; 71: 1168-1174