Highlights
- •Developmental validation of probabilistic genotyping software – STRmix™ NGS.
- •Demonstrate the software’s appropriateness to interpret NGS aSTR profiles.
- •Shows that sequence-based stutter models perform better than a length-based model.
- •Compares LRs assigned using sequence-based and length-based allele frequencies.
Abstract
We describe the developmental validation of the probabilistic genotyping software
– STRmix™ NGS – developed for the interpretation of forensic DNA profiles containing
autosomal STRs generated using next generation sequencing (NGS) also known as massively
parallel sequencing (MPS) technologies. Developmental validation was carried out in
accordance with the Scientific Working Group on DNA Analysis Methods (SWGDAM) Guidelines
for the Validation of Probabilistic Genotyping Systems and the International Society
for Forensic Genetics (ISFG) recommendations and included sensitivity and specificity
testing, accuracy, precision, and the interpretation of case-types samples. The results
of developmental validation demonstrate the appropriateness of the software for the
interpretation of profiles developed using NGS technology.
Keywords
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Article info
Publication history
Published online: November 08, 2022
Accepted:
November 7,
2022
Received in revised form:
November 4,
2022
Received:
August 29,
2022
Identification
Copyright
Crown Copyright © 2022 Published by Elsevier B.V. All rights reserved.
